Molecular Biology Services

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Molecular Biology Services Details

GENE SYNTHESIS

We are offering gene synthesis service at very reasonable price with a minimal turnaround time. The synthesized gene could be provided cloned in a commercially available vector or the vector of your choice. We can also provide the synthesized gene as linear DNA in lucrative price.
 
DNA EXTRACTION & PURIFICATION

We offer DNA Extraction and purification services for any size of DNA fragments, small to large quantity of plasmids and cosmids, and endotoxin-free if requested.
 
  • Mitochondrial DNA Isolation:
We offer mitochondrial DNA isolation from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies.
 
  • Genomic DNA Extraction:
We offer a range of genomic DNA extraction for sensitive, scalable purification from an expansive set of starting materials to maximize process efficiency and downstream performance. This includes a broad range of kits for purifying genomic DNA from a variety of samples including tissue, blood, cultured cell, plants, pathological samples, etc.
 
  • DNA Extraction from Metagenomic Samples:
Efficient extraction of DNA from all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical and mechanical disruption with specialized beads along with elimination of inhibitory compounds by precipitation using a novel cleanup buffer. Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis.
 


PLASMID AMPLIFICAION

We bring you a cost effective and fast way of amplifying low copy number plasmids based on your specifications.
 

ENDOTOXIN CLONING SERVICE

Dr. KPC Life Sciences Endotoxin Removal Service can effectively reduce the endotoxin level from the provided sample.
 

PCR OPTIMIZATION SERVICE

We offer primer designing, PCR optimization and consultancy services.
 
APOPTOTIC LADDER IDENTIFICATION

We provide easy and sensitive solution for detecting DNA fragmentation in apoptotic cells.
 
  
RNA SERVICES
 
  • Total RNA Extraction & Quality Check:
We can isolate total RNA from variety of starting materials like tissue, blood, cultured cell, plants, pathological samples, etc. We check the quality of the RNA by Bioanalyzer and Denaturing Gel Electrophoresis.
 
  • Small RNA Extraction & Quality Check:
We offer highly purified small RNA (<200nt) from different starting materials. We check the quality by two methods viz. Bioanalyzer and Denaturing Acrylamide Gel Electrophoresis.
 
  • In vitro transcription & Purification:
We provide purified RNA using in vitro transcription. One needs to provide the template, such as plasmids, PCR products or custom synthesized double stranded oligonucleotides.
 
  • cDNA Synthesis from Total RNA:
Dr. KPC Life Sciences can provide you cDNA synthesized from the given RNA using choice of primers.
 


GENE EXPRESSION SERVICES

We offer target gene expression analysis using real time PCR as well as by semi quantitative end point PCR.
 
TAQMAN PROBE
 
  • Taqman Probe Design and Synthesis:
We offer Taqman probe design services, aiming to save time, effort, and money from preventing complications.
 
  • Taqman Multiplex PCR optimization:
We are proud to offer a complete multiplex qPCR solution that enables you to perform upto a 4-plex reaction for gene expression and a duplex reaction for genotyping analysis.
 

LAMP PRIMER DESIGN


Dr. KPC Life Sciences designs primers for Loop-Mediated Isothermal Amplification (LAMP) assays. If required, on demand, we can standardize the LAMP reaction condition too.
 


CLONING SERVICES

Dr. KPC Life Sciences offers a full spectrum of Cloning Services to create custom DNA constructs.
 

SITE DIRECTED MUTAGENESIS (SDM) SERVICES

We offer site-directed mutagenesis service which introduces single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequences, quickly and economically. All final constructs are sequence verified and documented.
 


 

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